【摘要】 目的了解河北省淡水鱼中香港海鸥型菌的分布现状及耐药性。方法含头孢哌酮32μg/ml的血平板进行分离,API20NE和VITEK GNI+进行生化鉴定,药敏试验采用K-B琼脂纸片扩散法。结果 429份鱼类样本中,检出8株香港海鸥型菌,总检出率为1.86%(8/429),其中草鱼的检出率为5.43%(5/92),鲤鱼为0.53%(1/189),鲫鱼为2.08%(2/96),其他淡水鱼未检出(0/52)。药敏结果显示8株香港海鸥型菌对万古霉素、头孢哌酮、克林霉素、甲硝唑耐药,对多粘菌素B、替卡西林/克拉维酸、红霉素、庆大霉素、阿米卡星、四环素、环丙沙星、磺胺、氯霉素敏感。结论河北省淡水鱼存在香港海鸥型菌的污染并显示一定程度的耐药,应加强监测和预警,防止香港海鸥型菌病在河北省的流行和传播。更多还原

【Abstract】 Objective To study the contamination of freshwater fishes with Laribacter hongkongensis and its drug resistance in Hebei Province. Methods Laribacter hongkongensis was isolated by Mac-Lonkey Agar with 32μg cefoperazone/ml,and identified by API20NE and VITEK GNI+.Drug sensitive test was done by Kirdy-Bauer antibiotic testing. Results Eight strains of Laribacter hongkongensis were isolated from 429 freshwater fish samples,with the total detection rate of 1.86%(8/429).The positive rates in grass carp,carp,and crucian were 5.43%(5/92),0.53%(1/189),and 2.08%(2/96),respectively.But Laribacter hongkongensis was not detected in other freshwater fishes(0/52).Drug sensitive test showed that 8 strains of Laribacter hongkongensis were completely resistant to vancomycin,cefoperazone,clindamycin,and metronidazole;but they were sensitive to polymyxin B,ticarcillin/clavulanic acid,erythromycin,gentamicin,amikacin,tetracycline,ciprofloxacin,sulfa,and chloromycetin. Conclusions Laribacter hongkongensis needs to be particularly monitored in the freshwater fishes in Hebei Province.更多还原