【摘要】 目的了解2007-2008年湖南省流感病毒优势流行毒株A(H3N2)亚型的基因变异特征。方法取病原学监测中分离到的29株A(H3N2)亚型流感病毒毒株,对其血凝素基因进行逆转录-聚合酶链反应(RT-PCR),扩增产物纯化后测序;测序结果与WHO全球流感疫苗株序列进行同源比对,绘制种系发生树。结果2007年的分离株与2007-2008年北半球疫苗株A/Wisconsin/67/05比较,变异位点主要为G50E,S138A,K140I,R142G以及N144D。2008年分离株与北半球疫苗株A/Wisconsin/67/05比较,变异位点主要为G50E,S138A,K140I,L157S;而与2008-2009年北半球疫苗株A/Brisbane/10/07比较显示,变异位点主要为K140I,L157S。结论湖南省2007-2008年A(H3N2)亚型流感流行毒株的HA1基因发生了变异,可能是导致其流行的重要原因。更多还原

【Abstract】 Objective To investigate the molecular characterization of influenza A (H3N2) virus in prevalence in Hunan Province in 2007～2008. Methods Twenty-nine strains of influenza A (H3N2) virus were isolated from the samples in influenza surveillance. Hemagglutination genes of 29 strains were amplified by RT-PCR method. The RT-PCR products were purified and sequenced. And then the sequences were analyzed phylogenetically and compared with WHO influenza vaccine strains. Finally the phylogenetic tree was drawn according to deduced amino acid sequences. Results As compared with the northern hemisphere vaccine strain A/Wisconsin/67/05 in 2007～2008,the variation sites of influenza strains isolated in 2007 were G50E,S138A,K140I,R142G and N144D,and the variation sites of strains isolated in 2008 were G50E,S138A,K140I and L157S. But as compared with the northern hemisphere vaccine strain A/Brisbane/10/07 in 2008～2009,the variation sites of influenza strains in 2008 were K140I and L157SA. Conclusions HA1 gene variation of influenza A (H3N2) viruses in Hunan Province in 2007～2008 was recorded,which suggested it was the main cause leading to the spread of influenza.更多还原