【摘要】 目的了解常州地区单采献血人群中的抗丙型肝炎病毒(HCV)以及输血传播病毒(TTV)-DNA感染的状况,探讨TTV-DNA对单采血小板献血者对丙氨酸转氨酶(ALT)的影响。方法应用酶联免疫吸附试验(ELISA)法检测单采献血小板以及单一献全血人群中的抗-HCV;应用荧光定量聚合酶链反应(FQ-PCR)检测单采血小板献血者以及献全血者TTV-DNA;并应用全自动生化速率法检测TTV-DNA阳性及阴性献血者血清ALT。结果单采献血者抗-HCV、TTV-DNA的阳性率分别为0.15%、14.16%,普通献全血者则分别为0.43%、5.83%;和单一献全血者相比,单采血小板献血者抗-HCV阳性率低于献全血者(χ2=10.22,P<0.01);TTV-DNA阳性率则高于献全血者(χ2=6.57,P<0.05);单采献血者中TTV-DNA阳性和阴性献血者ALT水平分别为(22±7)、(17±10)U/L,两者比较差异无统计学意义(t=0.63,P>0.05)。结论单采献血小板者感染者抗-HCV低于单一献全血者,可能与其反复多次的检验有关;而单采献血者TTV-DNA感染者高于献全血者,可能和其频繁采血有关;单采献血者TTV-DNA阳性对单采献血者ALT无影响,其机制有待进一步的研究。更多还原

【Abstract】 Objective To investigate the rate of anti-HCV and the transfusion transmitted virus DNA(TTV-DNA)in platelet donors in Changzhou area,and to explore the relationship between TTV-DNA and ALT.Methods The anti-HCV antibody was measured by ELISA assay,and TTV-DNA was detected by FQ-PCR,and ALT was measured by auto biochemical method.Results The rate of anti-HCV antibody in platelet apheresis donors was 0.15%,it was lower than that of whole blood donors 0.43%(χ2=10.22,P<0.01).While the rate of TTV-DNA was 14.16%,and it was higher than that of whole blood donors 5.83%(χ2=6.57,P<0.05).There was no significant difference for ALT in serum between TTV-DNA positive platelet apheresis donors[(22±7)U/L] and TTV-DNA negative donors[(17±10)U/L](t=0.63,P> 0.05).Conclusion The rate of anti-HCV antibody in platelet apheresis donors was lower than that of whole blood donors,it was due to examining and reexamining.And the rate of TTV-DNA was higher than that of whole blood donors;there was no relationship between TTV-DNA and ALT level,but the underlying mechanisms are unknown.更多还原