【摘要】 目的分离鉴定板栗(Castanea mollissima Blume)总苞中抗糖尿病活性化合物。方法采用正-反相硅胶柱色谱和制备型HPLC进行分离,经理化常数测定、核磁共振技术分析等方法鉴定化合物的结构;对分离得到的单体化合物进行醛糖还原酶(aldose reductase,AR)抑制活性实验。结果从活性部位中分离得到9个化合物,分别为山柰酚(kaempferol,1)、没食子酸(gallic acid,2)、3,4-二羟基苯甲酸(3,4-dihydroxy-benzoic acid,3)、鞣花酸(ellagic acid,4)、苯甲基-6-O-β-D-吡喃葡萄糖苷(benzyl-O-β-D-glucopyranoside,5)、没食子酸正丁酯(n-butyl gallate,6)、2,4,6-三羟基苯甲酸(2,4,6-trihydroxy-benzoic acid,7)、没食子酸甲酯(methyl-gallate,8)、豆甾烷-4-烯-3,6-二酮(stig-mast-4-en-3,6-dione,9)。结论化合物5-7为首次从该属植物中分离得到,活性结果显示9个化合物均显示不同程度抑制活性,其中化合物4、5最为显著。更多还原

【Abstract】 Objective To study the anti-diabetic constituents of the involucre of Castanea mollissima Blume.Methods Compounds were isolated through various chromatography techniques.They were elucidated on the basis of physicochemical and NMR analysis,and their anti-diabetic activity were evaluated by AR inhibitory screening.Results Nine compounds were obtained and identified as kaempferol(1),gallic acid(2),3,4-dihydroxy-benzoic acid(3),ellagic acid(4),Benzyl-O-β-D-glucopyranoside(5),n-butyl gallate(6),2,4,6-trihydroxy-benzoic acid(7),methyl gallate(8),stigmast-4-en-3,6-dione(9),respectively.Conclusions Among them,compounds 57 are isolated from this genus for the first time.Nine compounds show the AR inhibitory activities with different degree,and compounds 4 and 5 exhibit much stronger inhibitory effects than positive control and other compounds.更多还原