【摘要】 目的研究环磷酰胺对大鼠脑出血血肿周围组织NF-κB表达和白细胞浸润的影响。方法健康雄性SD大鼠125只,随机分成3组:假手术组(25只)、对照组(50只)、治疗组(50只),采用Fredrik建立的自体股动脉取血并向大鼠尾壳核注射方法复制脑出血模型。各组按术后6,24,48,72h及5d分为5个时间点,经尾壳核行冠状切片,分别按干湿重法测脑含水量,免疫组织化学法测定NF-κB的表达,HE染色测定血肿周围组织白细胞浸润。结果假手术组各时间点NF-κB表达及白细胞浸润无明显变化。治疗组除6h、24h外,各时间点血肿周围脑组织含水量、白细胞浸润及NF-κB表达均低于对照组(P<0.05)。NF-κB表达与白细胞浸润呈现同步性,而且NF-κB的表达高峰时间先于血肿周围组织白细胞浸润。结论环磷酰胺具有减低NF-κB活性,抑制脑出血血肿周围组织的炎症反应起到神经保护作用。更多还原

【Abstract】 Objective To study the influence of cyclophosphamide on expressions of nuclear factor(NF)-κB and Leukocytic infiltration in the brain perihematoma region in rats with cerebral hemorrhage.Methods 125 health male rats were randomly divided into false-operation group(n=25),therapy group(n=50) and control group(n=50).Each group was further divided into 5 subgroups according to the time of 6,24,48,72 h and 5 d after model creation.The intracerebral hemorrhage model was duplicated with the method created by Fredrik.Brain water content was measured.Expressions of NF-κB and Leukocytic infiltration were assayed in each group by immunohistochemical method.Results The difference of NF-κB expression and Leukocytic infiltration was not significant in false operation group.Brain water content was lower in therapy group than that in control group(P<0.05);In the perihematoma region,positive cells of NF-κB and Leukocytic infiltration in therapy group were more than that in control group(P<0.05) except 6 h and 24 h.There was synchronism between express of NF-κB and express of Leukocytic infiltration.But the peak of NF-κB expression was earlier than that of Leukocytic infiltration.Conclusion Cyclophosphamide has the effect of brain protection through decreasing expressions of NF-κB and refraining inflammatory reaction.更多还原