【摘要】 目的探讨载脂蛋白E(ApoE)基因启动子-307～-7区单核苷酸多态性(SNP)与散发阿尔茨海默病(SAD)发病风险的关系。方法在36例SAD患者和36例正常人中,采用聚合酶链反应和变形梯度凝胶电泳检测再测序的方法,对ApoE基因启动子-307～-7区进行SNP的检测。结果ApoE基因启动子-307～-7区存在-219G/T多态,未发现新的SNP,SAD患者与正常对照组之间基因型频率分布无差异(P<0.05)。结论ApoE基因启动子-307～-7区未发现新的SNP,-219G/T多态与SAD无关。更多还原

【Abstract】 Objective To explore the association between single nucleotide polymorphism of the apolipoprotein E (ApoE) promoter spanning-307 to-7 and sporadic Alzheimer’s disease (SAD).Methods We detected single nucleotide polymorphism of the apolipoprotein E promoter spanning-307 to-7 in 36 SAD and 36 healthy subjects with polymerase chain reaction and denaturing gradient gel electrophoresis (DGGE) followed by sequencing.Results We found no distribution differences of the ApoE promoter-219 G/T polymorphism between SAD and controls (P<0.05),and no new single nucleotide polymorphism in the ApoE promoter spanning-307 to-7.Conclusion The ApoE promoter-219 G/T polymorphism is not correlated to the risk for SAD.更多还原