【摘要】 目的:通过体外实验研究钩藤酸E(UAE)抑制人肝癌细胞HepG2的细胞增殖作用,并进一步研究其作用机制。方法:MTT法考察钩藤酸E对人肝癌HepG2细胞的生长抑制作用,通过电镜观察细胞微细结构变化,流式细胞术进一步验证细胞凋亡,并检测细胞周期变化。结果:研究发现UAE能抑制HepG2细胞增殖。电镜分析证实UAE引起细胞凋亡。流式细胞术检测细胞凋亡主要发生于G0/G1期。结论:钩藤酸E诱导人肝癌细胞HepG2的细胞增殖,诱导其发生凋亡。更多还原

【Abstract】 Objective:To assess the effect of Uncarinic Acid E(UAE) on the HepG2 cell,a human hepatoma cell line,and investigate the mechanism of induced HepG2 cell death.Methods:Cytotoxic effect was examined by MTT assay;ultrastructure of HepG2 cell was observated by electronic microscopy;while the cell cycle distribution was determined by flow cytometric analysis.Results:Cell growth of HepG2 cells was inhibited by UAE,electron microscope analysis showed that UAE initiated apoptosis in HepG2 cell,which is also obtained by the result of flow cytometric analysis,and the cell cycle arrest occurred in G0/G1 phase.Conclusion:Uncarinic Acid E showed an anti-proliferation effect and induced apoptosis of HepG2 cell in vitro.更多还原