【摘要】 目的:通过研究地塞米松对大鼠肺泡巨噬细胞吞噬功能的影响,探讨地塞米松治疗急性肺损伤的作用机理。方法:健康雄性Wistar大鼠放血处死后全肺灌洗获取肺泡巨噬细胞(PAMs),贴壁纯化,在预培养20 h后重悬PAMs,分3组分别处理4 h。正常对照(C)组:不做任何干预;脂多糖(LPS)组:按10μg/mL的剂量加入LPS刺激;脂多糖加地塞米松(LPS+Dex)组:将含Dex终浓度1×10 mmol/L~1×6 mmol/L的RPMI 1640培养基加入LPS。收集PAMs,与1%鸡红细胞悬液培育后制片,瑞氏染色观察并计算吞噬率和吞噬指数,对两者做相关回归分析并采用单因素方差分析比较组间差异。结果:各组AM的吞噬率和吞噬指数呈线性相关性关系,从高到低依次为:C组(12.50±2.07)%,2.29±0.08;LPS+Dex组(8.25±1.67)%,1.73±0.18;LPS组(3.50±1.20)%,1.16±0.19;各组间的差异均具有统计学意义(P<0.05)。结论:LPS可抑制体外培养PAMs的吞噬活性;Dex对LPS抑制的PAMs吞噬活性有一定的活化作用。更多还原

【Abstract】 Objective: To investigate the mechanism of dexamethasone in treatment of acute lung injury by studying the effect of dexamethasone on phagocytic function of rat alveolar macrophages.Methods: After the healthy male Wistar rats were sacrificed to carry out whole lung lavage so as to take pulmonary alveolar macrophages and perform adherent purification,PAMs were resuspended after 20 h pre-culture and divided into 3 groups for intervention treatment for 4 hours respectively.The normal control(C) group was not interventioned.Lipopolysaccharide(LPS)group was stimulated by 10 μg/mL and LPS.LPS and Dex(LPS+Dex) group was treated by contained Dex final concentration 1×10 mmol/L～1×6 mmol/L RPMI 1 640 medium and LPS.PAMs were collected and incubated with 1% chicken erythrocyte suspension to make slice.Wright’s staining was implemented for calculating the ratio and index of phagocytosis.The two targets were treated with correlation-regression analysis and the disparity of 3 groups were compared with one-factor analysis of variance.Results: The phagocytic ratio and index of AM in each group showed linear correlation.The ratio and index of phagocytosis from descending order of the 3 groups were(12.50±2.07)%,2.29±0.08 in group C,(8.25±1.67)%,1.73±0.18 in group LPS and Dex and(3.50±1.20)%,1.16±0.19 in group LPS respectively.There were statistically significant differences between the 3 groups(P<0.05).Conclusion: LPS could inhibit the phagocytic activity of PAMs cultured in vitro.Dex has some activation for the PAMs phagocytic activity inhibited by LPS.更多还原