【摘要】 目的:以去势大鼠建立的绝经后骨质疏松模型,检测骨组织中晚期糖化终末产物(AGEs)含量,探讨其特异性抑制剂胍乙酸的防治效果。方法:54只SD大鼠随机分为三组:去势后给药(胍乙酸)实验组(groups1,G1),单纯去势组(G2)和假去势组(G3),并将术后第4,10和16周定为实验周。检测骨胶原组织中AGEs和骨形成指标:骨钙素(BGP),骨特异性碱性磷酸酶(骨AKP),骨吸收指标:尿吡啶醚(PYD)和脱氧吡啶醚(DPD),同步用IBAS计算机全自动图像分析系统对不脱钙骨组织动态观测骨形态计量学参数。结果:单纯去势后4～16周骨胶原AGEs含量明显高于假去势组;给予胍乙酸组明显低于去势组,而BGP和骨AKP胍乙酸组明显高于去势组。骨吸收指标:尿PYD和DPD两组之间无显著差异。骨形成表面(FS)和骨小梁体积(TBV),骨小梁平均厚度(MTT)较去势组明显升高,尤其TBV和FS,这种差异在第10周最为显著。随着给药时间的延长(第16周)MAR逐渐增加。骨吸收表面(RS)与单纯去势组比较在所有实验周均无差异。结论:去势后雌激素降低可致骨中AGEs增多,引发骨质疏松,而胍乙酸可通过降低AGEs刺激成骨细胞活跃增生,促进骨形成,改善骨组织形态。更多还原

【Abstract】 Objective:Exploring the role of advanced glycosylation end products(AGEs)pharmacal mechanism and in pathogenesis of ovariectomy induced osteoporosis,prevention for osteoporosis with glycocyamine.Methods:Fifty-four female Sprague-dawly rats were randomly divided into 3 groups:each group was administration treatment with glycocyamine(groups1,G1)and ovariectomized groups(G2)and sham-OV(G3).Laboratory weeks was hypothesized by with 4-16 weeks:After 4 weeks,10 weeks and 16 weeks of operation.Determining contents of AGEs from bone collagen and bone formation indexes:bone osteocalin(BGP);serum bone specific alkeliphosphatase(bone AKP),it was bone formation parameters,and bone resorption indices :pyridinolin(PYD)and deoxypyridinoline(DPD)were determined.At the same time,bone histomorphometry was measured with whole automobile imaging analysis system.Results:Contents of AGEs from ovariectomized rats(G2)bone collagen(58.96RU/mg)were higher than that from sham-OV(G3)(52.83RU/mg),contents of AGEs(51.33RU/mg)in administration with glycocyamine(G1)were lower than that from G2.By the 4-16 weeks after administration,BGP and bone AKP began to increase in G1,compared with G2 was improved significantly,but the PYD and DPD respectively level in urine were not significant decrease between in G1 than that from G2,and bony histomorphometry began to change gradually 4-16 weeks in G1,bone formation signal such as trabecular bone volume(TBV)and formation surfast(FS)of G1 increased significantly than those of G2,this difference was most obvious at 10 week.The increase of MAR was in a time-dependant manner.Conclusions:Increase AGEs contents from bone collagen in ovariectomized rats,the glycocyamine could stimulate active hyperplasia of bone cell and mainly influences for increased of bone formation which might prevent ovariectomized rats from bone loss by repressing the AGEs,lead to improve the shape of the bone in osteoporosis.更多还原