【摘要】 目的观察加味芍甘附子汤对Ⅱ型胶原诱导性关节炎(CIA)大鼠血清IL-6、IL-10含量的影响,并借以探讨本方治疗RA的作用机理。方法SD雄性大鼠50只随机分为空白组(A组)、模型组(B组)、甲氨喋呤组(C组)、尪痹颗罻?D组)、加味芍甘附子汤组(E组)。除A组外,其余4组分别给予每只大鼠尾根部皮内注射CⅡ乳剂0.1mL,10天后加强注射0.1mL,诱导成关节炎模型。从造模后第20天起开始给予相应药液灌胃,A、B组:清洁水,1次/日;C组:甲氨喋呤水溶液,1次/周;D组:尪痹药液,1次/日;E组:加味芍甘附子汤药液(1.5g/mL),1次/日。连续用药35天。用酶联免疫法测定其血清中IL-6、IL-10的含量。结果加味芍甘附子汤能明显降低CIA大鼠血清中IL-6的含量,与尪痹颗罻榧凹装钡首橄啾染邢灾圆钜?P<0.01);在提高CIA大鼠血清中IL-10的含量方面与甲氨蝶呤相似无明显差异(P>0.05);在改善大鼠全身及关节局部症状方面,治疗组及药物对照组均优于模型组,其中以加味芍甘附子汤组最为明显。结论加味芍甘附子汤能明显改善CIA大鼠的全身和关节局部症状,其作用机理可能与抑制血清IL-6、IL-10等炎症介质的产生有关。更多还原

【Abstract】 Objective:To observe the effect of of Jia wei shao gan fu zi tang on serum IL-6,IL-10 in CIA rats and to discuss mechanism of this formula treating RA.Methods:50 rats were randomly divided into five groups,blank(A)group,model(B)group,MTX(C)group,Wangbi Granule(D)group,and Jia wei shao gan fu zi tang(E)group.Except group A,rats of the rest four groups were injected with C II 0.1 ml,and ten days later injected with 0.1ml again to induce RA.20 days later started giving related medication i.g.A,B group:clean water,once a day;C group:MTX water solution;D group:Wangbi decoction;E group:Jia wei shao gan tang decoction 1.5 mg/ML,once a day,for 35 days.ELISA was used to test content of serum IL-6 and IL-10.Results:Jia wei shao gan tang lowered IL-6 of CIA rats obviously,and there was significant difference comparing with Wangbi Granule and MTX groups(P<0.01).There was no significant difference on increasing IL-10 comparing with MTX(P>0.05).Treatment group and medication controlling group improved general and joint local symptoms much better than model group and Jia wei shao gan tang was most obvious.Conclusion:Jai wei shao gan fu zi tang obviously improved general and joint local symptoms of CIA rats,the mechanism of which probably related to inhibiting mediators of inflammation such as IL-6 and IL-10.更多还原