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金属卟啉与刀豆蛋白A相互作用的室温磷光研究
Interaction between Metal-porphyrin and Concanavalin A by Room Temperature Phosphorescence
【摘要】 实验在脱氧胆酸钠盐(NaDC)介导下,采用室温磷光法研究阴离子meso-四-4-(羧基苯基)钯卟啉(Pd-TCPP)与刀豆蛋白A(ConA)之间的相互作用,实验表明Pd-TCPP与ConA之间能形成复合物,结合常数为3.33×104L·mol-1.这一结果可用于利用Pd-TCPP作为室温磷光探针对ConA进行测定.同时,紫外可见光谱和共振光散射光谱也同样证明Pd-TCPP与ConA之间存在相互作用.
【Abstract】 Room temperature phosphorescence was used to investigate the interaction between Pd-TCPP and ConA.RTP intensity of Pd-TCPP can be quenched by ConA which indicated an obvious interaction between two.The binding constant of this interaction was 3.33×104 L·mol-1.UV-Vis spectroscopy and resonance light scattering spectroscopy were also used to testify the conclusion.
【关键词】 meso-四-4-(羧基苯基)钯卟啉;
刀豆蛋白A;
室温磷光;
脱氧胆酸盐;
【Key words】 Pd-TCPP; ConA; room temperature phosphorescence; sodium deoxycholate;
【Key words】 Pd-TCPP; ConA; room temperature phosphorescence; sodium deoxycholate;
【基金】 国家自然科学基金(No.20475035);山西省自然科学基金
- 【文献出处】 山西大学学报(自然科学版) ,Journal of Shanxi University(Natural Science Edition) , 编辑部邮箱 ,2010年01期
- 【分类号】Q503
- 【被引频次】1
- 【下载频次】121