【摘要】 粘杆菌素由于其药性强、残留低、对人畜无害被认为是最安全的畜禽抗生素之一。利用微滤对粘杆菌素发酵液进行预处理,根据发酵液的特性,选择孔径为0.2μm、膜面积为0.06M2的管式陶瓷膜为微滤膜,研究了操作参数的适宜值:压力为0.2MPa,流量为20L/min,在浓缩倍数达到3.5倍时连续两次加入与浓缩液体等量的水,得率为96%。经微滤处理后,滤液的吸光度Abs(470nm)为0.394,N-NH3含量为115mg/100mL,有效地去除了菌体、胶体蛋白及部分色素等,效果优于工业生产中板框过滤滤液的质量标准。更多还原

【Abstract】 Colistin is one of the safest antibiotics to promote the growth of animals because of absorption in animal digestive tract and slight toxicity.Microfiltration was used to pretreat colistin fermentation broth.According to the characters of materials,inorganic ceramics membrane was selected.The best manipulation parameters were as follow: the pressure was 0.2MPa,the flow velocity was 20L/min.When the condense ratio reached to 3.5 times,the same volume water should be added.Under this parameter,OD(470nm) of permeated liquid was 0.394,the density of protein was 1.15mg/mL,and the yield of colistin was 96%.The pretreatment process of fermentation broth can be achieved by the application of microfiltration.It was more efficient than the existing plate filtration process.更多还原