【摘要】 使用动态光散射仪及微批量法,实时测量了溶菌酶晶体生长过程中,蛋白质颗粒的聚集情况。实验表明当液滴中的溶菌酶分子单体形式占多数,同时也存在一定数目的聚集体时,将会产生晶体。另外还通过观察有无絮状物附着情况下,溶菌酶晶体的生长情况,研究了絮状物对晶体生长速度的影响。实验表明这种由高密度的蛋白质聚集体组成的絮状物附着在晶体表面时,晶体的生长受到抑制。而絮状物会逐渐解体,重构成四方晶体或球状结晶等更稳定的聚集状态。研究在一定程度上揭示了溶液中溶菌酶分子的聚集状态与结晶的关系。更多还原

【Abstract】 In the processes of lysozyme crystal growth,the situations of protein aggregation were observed by Dynamic Light Scattering and microbatch method in real time.Experiment results showed when the majority of particle in the solution was lysozyme monomer,but there were some oligomer and polymer in solution,lysozyme tetragonal crystal was found in the drop.In addition,the effect of protein floc on crystal growth was also studied by measuring the growth rate of(110) face of tetragonal crystal absorbed by floc or not,respectively.The results showed that the floc made of high density lysozyme aggregate could restrain the growth of crystal when it adhered to the surface of crystal.When the floc dissolved gradually,it could build more stable form,such as tetragonal crystal or spherulitic crystal.This study partly reveals the relation between lysozyme aggregation and crystallization.更多还原