【摘要】 突触前释放的多巴胺以容积式释放(volume transmission)来传递多巴胺信号,而碳纤电极(carbon fiber electrode,CFE)所记录的胞外电化学信号是大量多巴胺分子氧化电流的平均统计结果。这些特征为使用Monte Carlo方法来直观而细致地描述多巴胺在胞外"自由行走"的行为提供了可能。作者尝试使用Monte Carlo建模的方法来研究动作电位(AP)刺激的频率对纹状体内多巴胺浓度([DA])变化的影响。结果显示,与实验记录多巴胺信号相比,在生理强度(低频≤20Hz,或≤12个APs高频80Hz)刺激模式下,基于单一囊泡库的突触模型可以较好地模拟实验结果。此外,作者还分析了大脑神经网络结构等对实验数据的影响。研究说明,Monte Carlo模拟为研究大脑内多巴胺信号的产生机制提供了一种有效的辅助研究方法。更多还原

【Abstract】 Dopamine,released from presynaptic terminal,transmit neuronal information through volume transmission.On the other hand,the dopamine signal,recorded by Carbon Fiber Electrode (CFE),is integration of tens of thousands of oxidized single molecule.Based on those properties,the authors investigate the effect of action potential (AP) stimulation frequency and pattern on dopamine signal with Monte Carlo simulation.It was found that,the simulation,based on single vesicle pool,can fit the experimental data following physiological strength of AP stimulations.Furthermore,the effects of the spatial structure of synaptic organization and the single electron’s signal on the dopamine signal are also studied.Collectively,the Monte Carlo simulation offers a good method for investigation of mechanisms of dopamine signal in the brain.更多还原