【摘要】 利用同源序列法克隆了中山大蕉中的MuNPR1-1和MePR-1基因,RT-PCR分析了MuNPR1-1和MePR-1基因对香蕉枯萎病4号小种的应答反应,结果表明抗病的中山大蕉幼苗叶片经香蕉枯萎病4号小种接菌诱导后,MuNPR1-1基因的表达水平在诱导后12 h达到最高,高表达持续到24 h,在72 h降到接近起始状态,病原相关蛋白MePR-1基因的表达在24 h达到最高,48 h开始下降,72 h又恢复到起始水平。而感病的粉蕉中MuNPR1-1在0、12和24 h无明显变化,在48-72 h表达增加,病原相关蛋白MePR-1基因的表达在0、12和24 h无明显变化,在48-72 h有增加,但表达强度低于中山大蕉。这表明了抗病的中山大蕉的MuNPR1-1基因对病原菌信号分子的反应比感病品种粉蕉敏感,有助于激活下游防卫基因的表达。更多还原

【Abstract】 We cloned the full-length cDNA of MuNPR1-1 and MePR-1 gene using homologous cloning and RACE techniques from Zhongshandajiao.RT-PCR is used to analyze the expression changes of MuNPR1-1 and MePR-1 in Zhongshandajiao and Fenjiao after FOC 4 treatment.The results showed that MuNPR1-1 expression level reach the highest point in 12 h and MePR-1 in 24 h after FOC 4 treatment,while after FOC 4 treatment for 72 h,the expression level is just as non-treated.However,the MuNPR1-1 and MePR-1 from susceptible-Fenjiao have no distinct changes until 48 h after treatment,and the highest level last to 72 h.It indicated that the MuNPR1-1 of resistant-Zhongshandajiao show more sensitive to pathogen signal molecules than that of susceptible-Fenjiao.This sensitivity to pathogen might be favorable to express downstream plant defense genes.更多还原