【摘要】 采用基因重组的方法构建了含有猪传染性胃肠炎病毒S基因B和C抗原位点片段的巴斯德毕赤酵母Pichiapastoris分泌型表达载体pPIC9K-ts,经线性化后采用电穿孔法将其导入毕赤酵母GS115中,大量筛选后获得高效表达外源蛋白的毕赤酵母工程菌株GS115/pPIC9K-ts。表达蛋白经Dot-ELISA检测具有良好的抗原性。本研究为TGE的血清学检测方法的建立提供了必要的物质基础。更多还原

【Abstract】 A DNA fragment coding for TGEV spike gene B and C antigen sites was cloned into P.pastoris expression vector pPIC9K for extracellular expression in P.pastoris by gene recombination.The resulted recombinant plasmid pPIC9-ts was then linearized and transformed into P.pastoris GS115 by electroporation.The ts gene was integrated into the genome of P.pastoris through homologous recombination and an expression strain,named GS115/pPIC9K-ts,was obtained.Dot-ELISA showed that the recombinant protein possessed favourable antigenicity.This research provided essential material foundation for establishment of TGE serology diagnostic method.更多还原