【摘要】 研究超低温方法保存油菜花粉过程中的预冻和解冻处理方式对油菜花粉的形态、大小及其活力的影响。结果表明:采用0℃(12h)→-4℃(12h)→-20℃(12h)→-80℃(12h)变温预冻处理和用-80℃(1h)→-20℃(1h)→4℃(1h)逐步解冻后对油菜花粉形态大小和活力影响最小。而经过25℃室温解冻法和42℃快速解冻法处理后油菜花粉出现破裂,破裂率分别达到7.6%和9.1%。同时液氮保存油菜花粉的时间长短对花粉的大小及活力影响不大。更多还原

【Abstract】 Effect of pre-freezing and unfreezing treatment on the size and activity of rapes pollens was studied under ultra low-temperature condition.The results showed that the lowest effect on were those by pre-freezing treatment with gradually falling-temperature [0℃(12 h)→4℃(12 h)→-20℃(12 h)→-80℃(12 h)] and unfreezing treatment with gradually increasing-temperature [-80℃(1 h)→-20℃(1 h)→4℃(1 h)].The pollens un-frozen at room temperature(25℃)and at 42℃ were partly broken with the rates of 9.1% and 7.6%,respectively.Moreover,storage time in liquid nitrogen was not significant to the size and activity of the pollens.更多还原