【摘要】 目的:对羊血进行提取SOD。方法:采用有机溶剂去除血红蛋白、热变性、丙酮沉淀、DEAE-32离子交换柱层析的方法,对羊红细胞Cu,Zn-SOD进行分离纯化。利用非变性凝胶电泳NBT活性染色鉴定SOD,SDS-PAGE测定分子量。结果:表明1 000ml羊血中得到SOD干粉1 257mg,总活力为79 453U,比活力为3 871.4U/mg。活性染色结果证明羊血SOD有2条带,表明已达到电泳纯。SDS-PAGE测得SOD亚基分子量分别为16.71kDa、15.97kDa。H2O2对羊血CuZn-SOD活性有抑制作用,通过紫外光谱扫描,羊血SOD在231nm处有最大吸收峰。更多还原

【Abstract】 Objective:To extraet SOD from goat blood.Method: Cu,Zn-SOD was purified from goat blood by organic solvent to remove hemoglobinn,thermal denaturalization,super-filtration condensation,acetone precipitation and DEAE-32 ion-exchange chromatography.using non-denaturing gel electrophoresis,stained with NBT activity and its molecular weight was analyzed by the SDS-PAGE.Result:The 1 000ml goat blood has been SOD powder 1 257mg,the total activity was 79 453U,specific activity was 3 871.4U/mg·pr.Electrophoresis showed that SOD has two SOD activity bands.Through SDS-PAGE electrophoresis determination of the two SOD subunits proteins with molecular weight were 16.71kDa,15.97kDa.The purified goat blood SOD activity inhibition by H2O2,Through UV scanning,there was goat blood SOD in the 230.5nm absorption peak.更多还原