【摘要】 介绍一个植物表达系统,由热激蛋白基因(AtHsp17.6-C2)的启动子来驱动GUS基因的表达。在22℃生长条件下,稳定遗传的转基因植株中几乎检测不到GUS的活性。但是当温度升至34～37℃时,GUS的活性迅速升高。37℃是该植物表达系统最适诱导温度。转基因植株经37℃热诱导2小时后再返回22℃培育2小时,GUS的活性增加80多倍。多次热诱导实验表明这个表达系统是能够被重复多次热诱导的。实验结果表明这个植物诱导表达系统能够适用于多种目的需要。更多还原

【Abstract】 A gene expression system were constructed,driven by a heat-shock gene promoter(AtHsp17.6-C2),to control the expression of reporter gene(GUS)in transgenic plants.The expression of the AtHsp17.6-C2 chimeric gene in the stable transformants of ⊿F1-22-13 was hardly detected in culture at 22℃.However,the expression increased dramatically at the transcriptional level when the incubation temperature was shifted to 34~37℃.The optimal temperature for heat(shock)induction was 37℃.After a 2h incubation at 37℃ and 2h recovery phase at 22℃,GUS activity was about 80 fold greater than that before heat shock.Multiple heat-shock treatments showed that this system was heat reinducible.This new system can be applied in numerous and various applications.更多还原