【摘要】 为利用大孔吸附树脂分离纯化三孢布拉霉菌发酵液番茄红素,考察了4种大孔树脂(HP20,HP2MG,SP825,SP207)对番茄红素的吸附-解吸特性,筛选出最佳树脂。结果表明,HP20大孔树脂对番茄红素具有较好的吸附-解吸性能,且吸附迅速,能在1h内达到吸附平衡。用含55%和60%异丙醇的丙酮不连续梯度洗脱,回收率可达89.55%,重结晶后可获得纯度95%以上的番茄红素,经红外、质谱、核磁等鉴定为天然型番茄红素。该方法简单,可操作性强,极具工业应用价值。更多还原

【Abstract】 The potential of macroporous resin for separation and purification of lycopene produced by B.trispora were investigated.The adsorption-desorption properties for lycopene of the resins such as HP20,HP2MG,SP825 and SP207 were studied.The optimal resin for lycopene was screened and selected.The results expressed that HP20 resin had a better adsorption-desorption property for lycopene.It could quickly reach an adsorption balance in one hour.Purification of lycopene using packed bed column by HP20 resin was carried out using step gradient of 55% isopropyl alcohol in acetone followed by 65% isopropyl alcohol in acetone.A 89.55% recovery of lycopene was detected by HPLC.After further recrystallisation the purity of lycopene was above 95% and the structure was confirmed by IR,MS and 1H-NMR.The whole process was simple,easy to operate,with a good industrial application value.更多还原