【摘要】 特定电场参数的电脉冲被广泛应用于生物医学领域中。传统的电穿孔常用于细胞融合、转染、电化学疗法等。近年来出现了细胞内电处理的应用,即电场强度为MV/cm、脉宽达到10 ns级的电脉冲能绕过细胞膜影响细胞内信号转导与胞内结构。研究表明,在以上两者的电参数范围之间存在致使癌细胞发生不可逆性凋亡的电场参数,这种介于电穿孔和细胞内电处理的过程即为不可逆性电击穿。不可逆性电击穿通过诱导细胞凋亡而选择性地杀死肿瘤细胞,可能为癌症治疗提供了一种新的方法。更多还原

【Abstract】 Depending on the specified electric field parameters such as electrical field strength and duration,electrical pulses have generally been applied in biomedical fields.Conventional electroporation is commonly used for cell fusion,transfection,electrochemotherapy and so on.Recently,pulse electric fields with high strength(MV/cm) and duration of several tens of nanoseconds can affect intracellular signal transduction and intracellular structures with plasma intact,owing to the application of intracellular manipulation.Most researches indicate that parameters between those two ranges could be used to result in an inreversible apoptosis of cancer cells.The procedure between electroporation and intracellular manipulation is irreversible electrical breakdown(IREB).IREB selectively kills tumor cells by inducing cell apoptosis;thus it might provide a new method for cancer therapy.更多还原