【摘要】 以海藻酸钠为载体,采用交联吸附法固定果胶酶。通过单因素实验和正交实验考察固定化主要因素对固定化酶活力的影响,优化固定条件。结果表明,在海藻酸钠浓度为2%、氯化钙为1%、戊二醛浓度为3%的条件下,采用酶浓度为0.2mg/mL、pH值3.0、温度为40℃、固定时间为45min。固定化果胶酶活力最高,酶活回收率达到84.4%。更多还原

【Abstract】 In this experiment,taking alginate as the carrier,pectinase was immobilized through crosslinking-absorption method.The influence of main immobilization elements put on the activity of immobilized enzyme was tested,and the fixed conditions was optimized through single-element experiment and orthogonal experiment.The results indicates that,under the density conditions of 2% alginate,1% calcium chloride and 3% glutaric dialdehyde,the highest activity of immobilized pectinase is found,whose reclaimed rate reaches to 84.4%,with 0.2 mg/mL enzyme density,3.0 pH,40 ℃ temperature and the fixed 45 minutes.更多还原