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反相高效液相色谱法对猪肉组织中恩诺沙星和环丙沙星残留量检测的改进

Improved Study on the Determination of Enrofloxacin and Ciprofloxacin Residue in Pork Tissue by Reversed Phase HPLC

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【作者】 孙焕赵榕牛晋阳郭文萍

【Author】 SUN Huan, ZHAO Rong, NIU Jin-yang, GUO Wen-ping (China Meat Research Center,Beijing 100068,China)

【机构】 中国肉类食品综合研究中心

【摘要】 利用配有荧光检测器的高效液相色谱同时分析动物组织中恩诺沙星及其代谢产物环丙沙星的含量。研究结果表明:环丙沙星、恩诺沙星的质量浓度在2μg/L~100μg/L范围内呈线性关系,相关系数分别为0.9993和0.9991,该方法中恩诺沙星和环丙沙星的最低定量限均为2μg/kg(S/N=10),恩诺沙星的回收率为85%~90%,环丙沙星的回收率为92%~99%。

【Abstract】 Enrofloxacin and ciprofloxacin residues in animal tissue were determined simultaneously by HPLC with f1uorescence detector. The conclusion showed that the linear relationship were kept in the range of 2 μg/L-100 μg/L, with correlation coefficients 0.999 3 and 0.999 1 respectively. The minimum quantitative limit of enrofloxacin and ciprofloxacin were both 2 μg/kg. The recovery of enrofloxacin was between 85 %-90 % and the recovery of ciprofloxacin was between 92 %-99 %.

【关键词】 恩诺沙星环丙沙星高效液相色谱法残留
【Key words】 enrofloxacinciprofloxacinHPLCresidues
  • 【文献出处】 食品研究与开发 ,Food Research and Development , 编辑部邮箱 ,2010年04期
  • 【分类号】TS251.7
  • 【被引频次】6
  • 【下载频次】257
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