【摘要】 为鉴别不同地区的普洱茶晒青毛茶,对31个来自不同地区的普洱茶晒青毛茶进行AFLP-毛细管电泳技术分析,一共得到426个可重复的位点,多态位点百分率为94.6%,聚类分析主要分为台地茶类、老树茶类等两大类。结果表明,采用以下方法可鉴别不同来源的普洱茶晒青毛茶:从典型的主要特征峰和染色信号的最高值和次高值来区别;从峰形图的整个形状来区分;与对照的峰形图比较来区分。表明AFLP-毛细管电泳技术是一种高效的分子标记技术,能成功应用于不同区域普洱茶晒青毛茶的鉴别。更多还原

【Abstract】 Amplified fragment length polymorphisms (AFLPs) markers were employed to identify the geographical origins of 31 sundried tea samples, which was a material for Pu erh tea processing. A total of 420 polymorphic bands out of 426 reproducible products were amplified. Percentage of polymorphic loci (P) was 94.6%. Dendrogram generated after UPGMA clustering by NTSYS software indicated that 31 samples could be divided into conventional and ancient tea type. There were three methods to identify ancient tea and conventional tea, and to discriminate ancient tea from other geographical origins using AFLP marker: (1) based on the main peak value and the highest and second highest value of dye signal of tea samples. (2) based on the whole AFLP diagram of different samples. (3) cmpare target tea AFLP diagram with control tea group AFLP diagram. The result showed that AFLP marker was an effective tool and a robust method in discrimination of sun-dried tea (Maocha).更多还原