【摘要】 目的:分离纯化核桃隔膜多糖并分析其单糖组成。方法:核桃隔膜经水提醇沉、冷冻干燥得水溶性多糖,经DEAE-纤维素52离子柱和SephadexG-100凝胶柱分离纯化后得到多糖组分PDJL-A11(diaphragma of juglans regia L.acid polysaccharide)。运用紫外光谱鉴定纯度,气相色谱分析多糖的单糖组成。结果:核桃隔膜多糖PDJL-A11单糖组成为:阿拉伯糖、果糖、甘露糖、鼠李糖和葡萄糖,其物质的量比为2.11:6.52:8.81:12.59:15.58。结论:建立了核桃隔膜多糖分离纯化的方法,确定了其单糖组成及其物质的量比、可为核桃隔膜药理学的研究提供参考。更多还原

【Abstract】 The purpose of this work was to isolate and purify polysaccharide from diaphragma of Juglans regia L(PDJL) and analyze its monosaccharide composition.A water-soluble crude polysaccharide was obtained by water extraction,ethanol precipitation and freeze-drying.The crude polysaccharide was purified to obtain a fraction of PDJL-A11 on DEAE-cellulose52 column and SephadexG-100 column.The purity of PDJL-A11 was analyzed by UV spectrophotometer.The monosaccharide composition and molecular ratio of PDJL-A11 were analyzed by gas chromatography.The monosaccharide composition of PDJLA11 was composed of arabinose,fructose,mannose,rhamnose and glucose with a molar ratio of 2.11:6.52:8.81:12.59:15.58.更多还原