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海水和淡水养殖花鲈肌肉脂肪酸组成和含量分析

Fatty Acid Composition of the Muscle of Lateolabrax japonicus (Cuvier) Grown in Seawater and Freshwater

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【作者】 许建和徐加涛林永健罗刚毕祥静

【Author】 XU Jian-he1,XU Jia-tao1,LIN Yong-jian2,LUO Gang1,BI Xiang-jing1(1.School of Ocean,Huaihai Institute of Technology,Lianyungang 222000,China;2.Ningbo Academy of Ocean and Fishery,Ningbo 315010,China)

【机构】 淮海工学院海洋学院宁波市海洋与渔业研究院

【摘要】 采用Folch液回流提取投喂相同配合饲料的海水养殖花鲈和淡水养殖花鲈背部肌肉脂质,脂质经氢氧化钾-三氟化硼法甲酯化后进行气相色谱分析。结果表明:海水养殖花鲈和淡水养殖花鲈肌肉脂肪酸组成存在一定差异。海水花鲈肌肉脂肪酸中单不饱和脂肪酸(MUFA)、n-3系列多不饱和脂肪酸(PUFA)、EPA(C20:5n-3)和DHA(C22:6n-3)含量明显高于淡水花鲈,而饱和脂肪酸(SFA)、n-6系列PUFA和AA(C20:4n-6)含量则低于淡水养殖花鲈。海水养殖花鲈和淡水养殖花鲈肌肉中n-6和n-3系列PUFA比值分别为0.27和0.42,均远低于HMSO(英国卫生部)推荐的最高安全限值(4.0)。

【Abstract】 Lipids in the dorsal muscle of Japanese seabass Lateolabrax japonicus grown in seawater and freshwater fortified with the same feed were extracted by reflux extraction with "Folch" solvent,esterified with KOH-BF3 method and analyzed by gas chromatography.The results indicated that an obvious difference in fatty acid profile was observed between the muscles of Japanese sea basses grown in seawater and freshwater.The muscle of Japanese seabass from seawater exhibited higher contents of monounsaturated fatty acids (MUFA),n-3 polyunsaturated fatty acids (PUFA),eicosapentaenoic acids (EPA,C20:5 n-3) and docosahexaenoic acids (DHA,C22:6 n-3),and lower levels of saturated fatty acids (SFA),n-6 PUFA and arachidonic acids (AA,C20:4 n-6).The ratios of n-6/n-3 fatty acids in the muscles of seawater and freshwater sea basses were 0.27 and 0.42,respectively.both significantly lower than maximum safety standard (4.0) recommended by the UK Department of Health.

【关键词】 花鲈脂肪酸海水淡水
【Key words】 Japanese sea bass Lateolabrax japonicusfatty acidsseawaterfreshwater
【基金】 江苏省企业博士创新基金项目(BK2009652);江苏省海洋生物重点实验室开放课题(2007HS001)
  • 【分类号】S917.4
  • 【被引频次】15
  • 【下载频次】313
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