【摘要】 建立阳离子交换-液相色谱测定鹿茸中唾液酸含量的方法。确定用盐酸对试样进行水解处理。色谱条件:色谱柱为Zorbax300-SCX阳离子交换柱,流动相为乙腈-0.1%磷酸水溶液(90:10,V/V),流速1.0mL/min,进样量10.0μL,紫外检测波长205nm。结果表明,鹿茸中的唾液酸质量浓度在5～100μg/mL范围内与峰面积线性关系良好,最低检测限浓度(RSN=3)为0.60μg/mL,方法检出限为0.03g/kg,平均加样回收率为83.4%,RSD为4.9%(n=6)。此法适用于鹿茸及相关产品中唾液酸含量的检测。更多还原

【Abstract】 A cationic exchange-HPLC method was presented for determination of sialic acid in antler velvets.Hydrochloric acid was used to hydrolyze samples.The optimum determination conditions based on the use of ZORBAX 300-SCX cationic exchange column were determined as follows:mobile phase,a mixture of acetonitrile and 0.1% phosphoric acid(90:10,V/V) at a flow rate of 1.0 mL/min;sample loading amount,10.0 μL;and detection wavelength,205 nm.There was good linearity between peak area and sialic acid concentration within the range from 5 to 100μg/mL.The detection limit(RSN = 3) of the method developed was 0.03 g/kg for sialic acid.The average recovery for sialic acid was 83.4%,with a RSD of 4.9%(n = 6).This method is applicable for the determination of sialic acid in antler velvets and antler velvet products.更多还原