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吹扫捕集GC-MS法测定紫苏不同变种叶片中的挥发性成分

Purge and Trap GC-MS for the Identification of Volatile Composition of the Leaves of Different Variants of Perilla L.

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【作者】 胡彦丁友芳温春秀谢晓亮周巧梅刘玉军

【Author】 HU Yan1,2,DING You-fang1,WEN Chun-xiu3,XIE Xiao-liang3,ZHOU Qiao-mei3,LIU Yu-jun1,*(1.College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,China ;2.Department of Biochemistry,Wenshan University,Wenshan 663000,China;3.Medicinal Herbs Research Center,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051,China)

【机构】 北京林业大学生物科学与技术学院文山学院生化系河北省农林科学院药用植物研究中心

【摘要】 采用吹扫捕集技术对紫苏不同变种叶片中的挥发性成分进行富集,以气质联用仪(GC-MS)进行鉴定,运用面积归一化法确定各组分的相对含量。结果表明,分别属于紫苏属植物3个变种的4份试材之间叶片挥发油的组分和相对含量差异显著。4份紫苏试材共检测出31种挥发油成分,但其共有的成分仅有顺-3-己烯醛、石竹烯和圣亚麻三烯3种。野生紫苏变种的主要成分是柠檬烯(77.90%)、石竹烯(14.33%)、顺-3-己烯醛(4.9%)、胡椒酮(1.71%),回回苏变种的主要成分是石竹烯(30.65%)、柠檬烯(24.60%)、顺-3-己烯醛(21.82%)、紫苏醛(17.70%),紫苏变种1(来源于河北安国市)的主要成分是5-乙叉-1-甲基-环庚烯(52.79%)、顺-3-己烯醛(19.98%)、石竹烯(13.60%)、紫苏醛(9.46%),紫苏变种2(来源于广西灵山县)的主要成分是紫苏醛(31.86%)、5-乙叉-1-甲基-环庚烯(31.68%)、顺-3-己烯醛(21.50%)、石竹烯(10.47%)。

【Abstract】 Purge and trap technique was used for enriching the volatile components of the leaves of different variants of Perilla L.prior to GC-MS analysis.Area normalization method was employed for the subsequent quantification.The results showed that there were significant differences in type and relative contents of volatile components among 4 samples belonging to 3 variants of Perilla L..Totally 31 volatile components were identified in the samples,3 of which,namely [Z]3-hexenal,caryophyllene and santolina triene were contained in all of the samples.The main components of the leaves of wild growing P.frutescens(Linn.) Britt.var.acuta(Thunb.) Kudo included limonene(77.90%),caryophyllene(14.33%),[Z]-3-hexenal(4.9%),and 3-methyl-6-[1-methethyl]-2-cyclohexen-1-one(1.71%),those of the leaves of P.frutescens(Linn.) Britt.var.crispa(Benth.)H.W.Li caryophyllene(30.65%),limonene(24.60%),[Z]-3-hexenal(21.82%) and perilla aldehyde(17.70%),and those of the sample No.1 of P.frutescens(Linn.)Britt.var.frutescen from Anguo,Hebei province 5-ethylidene-1-methyl-cycloheptene(52.79%),[Z]-3-hexenal(19.98%),caryophyllene(13.60%) and perilla aldehyde(9.46%),and those of the sample No.2 of P.frutescens(Linn.)Britt.var.frutescen from Linshan,Guangxi autonomous region perilla aldehyde(31.86%),5-ethylidene-1-methyl-cycloheptene(31.68%),[Z]-3-hexenal(21.50%) and caryophyllene(10.47%).

【基金】 河北省科技攻关项目(07276410D);“十一五”国家科技支撑计划重大专项(2009ZX09308-002)
  • 【分类号】R284
  • 【被引频次】25
  • 【下载频次】433
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