【摘要】 对水菖蒲内生真菌进行分离,并进行抗菌活性的筛选。采用改良氯化三苯四氮唑显色(TTC)微量稀释法筛选抗菌活性菌株。以18种常见导致食品腐败的细菌、酵母菌和霉菌为指示菌研究活性菌株的抗菌能力。结果显示,水菖蒲内存在广泛的具有抗菌活性的内生菌,占内生菌总数的40.7%,高活性菌株占内生菌总数的12.39%。其中S9菌株发酵液具有抗细菌、酵母菌和丝状真菌的广谱抗菌能力,是潜在的食品防腐抗菌资源。更多还原

【Abstract】 In order to isolate and screen endophytic fungi with inhibitory activity from Acorus calamus L.,active strains were screened through modified TTC micro-dilution method.MIC(minimum inhibitory concentration) and MBC(minimum bactericidal concentration) were detected using 18 food spoilage strains.Results indicated that 40.7% endophytes exhibited antimicrobial activity and 12.39% endophytes exhibited higher antimicrobial activity.In addition,strain S9 exhibited the broadspectrum antimicrobial activity to bacteria,yeast and mycelia fungi.Active endophytic strains from A.calamus L.are potential resources for food preservation.更多还原