【摘要】 目的:研究大球盖菇提取物(SRE)对小鼠肝和主要脏器的抗氧化酶活性及同工酶的影响。方法:小鼠连续30d给予低(100mg/(kgbw·d))、中(200mg/(kgbw·d))、高(400mg/(kgbw·d))剂量SRE后,灌胃给予CCl4(80mg/(kgbw·d))建立动物急性肝损伤模型,动物处死后取肝及主要脏器测定抗氧化酶活性,采用聚丙烯酰胺梯度凝胶电泳分析CAT和SOD同工酶。结果:与模型组比,高剂量SRE能显著提高肝总SOD、Mn-SOD、CuZn-SOD及CAT、GPx活性。主要脏器抗氧化酶活性测定表明,给予SRE后,心和肾SOD、CAT、GPx活性比模型组显著提高;造模后肾Mn-SOD表现出代偿性增高,给予SRE后能使其恢复到对照组水平。各脏器CAT同工酶都为1条,在造模后酶带染色密度明显降低,在给予SRE后能恢复到正常水平。肝有1条Mn-SOD带和3条CuZn-SOD带,在给予SRE后能使这些酶蛋白含量达到或高于正常水平;心、肾有1条Mn-SOD带和4条CuZn-SOD带,SRE能增加心、肾CuZn-SOD酶蛋白染色密度。结论:SRE能通过提高抗氧化酶的活性和同工酶酶蛋白含量,有效预防急性肝损伤小鼠肝脏和主要脏器的氧化损伤作用。更多还原

【Abstract】 Objective:To evaluate the effect of Stropharia rugoso-annulata extract(SRE) on antioxidative enzyme activity and isozyme profile in liver and other major visceral organs of mice with CCl4-induced acute liver injury.Methods:After 4 days of normal feeding,CIR female mice(25 ± 2) g were used as experimental animals.Ten CIR mice orally administered distilled water were served as control group.Fifty-six more CIR mice were randomly divided into 4 groups of 14 mice each:SRE groups orally administered SRE solution in distilled water at low(100 mg/(kg bw·d)),medium(200 mg/(kg bw·d)) and high(400 mg/(kg bw·d)) doses and model group orally administered distilled water.All mice were administered for 30 consecutive days and low-,medium-and high-dose SRE groups and model group were fed water fasting diet for 16 h on the 30th day before oral administration of CCl4 dispersed in salad oil for creating CCl4-induced acute liver injury model.At 24 h postadministration,all mice were sacrificed to assay activities of antioxidant enzymes in liver,heart,kidney and spleen and analyze isozyme profile of CAT and SOD by gradient polyacrylamide gel electrophoresis.Results:Compared with the model group,high-dose SRE group exhibited significantly increased activities of total SOD,Mn-SOD,CuZn-SOD,GPx and CAT in liver;In addition,there was a significant increase of activities of SOD,CAT and GPx in heart and kidney in three SRE groups.Compared with the normal group,the model group exhibited a compensatory increase of Mn-SOD in kidney;however,Mn-SOD activity was decreased to the normal group level after SRE administration.One CAT band was detected in liver,kidney and heart and CCl4-induced acute liver injury resulted in a significant decrease of staining intensity of the brand.In contrast,the staining intensity of the band was increased to the normal group level after SRE administration.Similarly,one Mn-SOD and three CuZn-SOD bands were detected in liver and the staining intensity of the isozymes was also increased to the normal group level after SRE administration.One Mn-SOD and four CuZn-SOD bands were detected in heart and kidney and SRE administration resulted in the increase of staining intensity of CuZn-SOD bands.Conclusion:SRE can protect liver and other major visceral organs of mice with CCl4-induced acute oxidative injury by enhancing the activities of antioxidant enzymes and the contents of isozymes.更多还原