【摘要】 建立食用菌中三聚氰胺的固相萃取-高效液相色谱检测法。样品经三氯乙酸、乙腈提取,离心,混合型固相萃取小柱净化后,过0.45μm滤膜,用配有二极管阵列检测器(PAD)的液相色谱仪检测,外标法定量。同时,以三聚氰胺标准品进行添加回收率测定,结果显示,本方法对三聚氰胺的测定低限为2.0mg/kg,回收率为81.3%～91.7%,测定的相对标准偏差均不大于5.6%。本方法能满足食用菌中三聚氰胺残留量常规检测的需要。更多还原

【Abstract】 A novel method based on solid phase extraction followed by high performance liquid chromatography(HPLC) was developed for the analysis of melamine residue in edible fungi.Samples were extracted with a mixed system composed of trichloroacetic acid and acetonitrile(3:1,V/V),cleaned up on mixed-mode SPE column and filtrated through 0.45 μm pore size membrane prior to analysis using a HPLC system equipped with PAD.Melamine was quantified by external standard method.The method exhibited a limit of detection of 2.0 mg/kg and a spike recovery range of 81.3% to 91.7% at three levels,with relative standard deviations(RSDs) of not more than 5.6%.The method proves to be most suitable for the determination of melamine residue in edible fungi.更多还原