【摘要】 建立猪肉中类固醇类激素残留高效液相色谱-串联质谱(LC-MS/MS)分析方法。样品经甲醇提取,固相萃取柱净化后采用LC-MS/MS进行检测分析;并对样品前处理条件和质谱参数进行研究。结果表明:这些激素物质的线性范围均为0.5～100μg/kg;低、中、高3种加标水平的加标回收率为71%～89%;最低检出限为0.2～2μg/kg。本方法快速简便、灵敏度高、选择性好、测定结果令人满意,可在各食品检测机构推广应用。更多还原

【Abstract】 A method using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was proposed for the determination of steroid hormone multi-residues in pork.Analytes were extracted from samples with methanol and cleaned up using a solid-phase extraction cartridge prior to HPLC-MS/MS analysis.The proposed method exhibited a good linearity for 10 steroid hormones over the range of 0.5-100μg/kg and a limit of detection ranging from 0.2 to 2μg/kg.Mean recoveries for 10 steroid hormones in blank pork samples spiked at low,medium and high concentrations were between 71% and 88%,with relative standard deviations below 10%.The method is characteristic of rapidity,simplicity,good selectivity and high sensitivity,and therefore has a great popularization potential.更多还原