【摘要】 建立了一套检测食品中杀草强的液相色谱及液相色谱-质谱分析方法,采用高效液相色谱荧光检测器(LC-FLD),在激发波长380nm、发射波长484nm条件下,高效液相色谱串联四级杆质谱配大气压电离源(LC-MS/MS-APCI),在多反应监测(MRM)模式下,母离子m/z85、定量子离子m/z43、定性子离子m/z57、碰撞能量m/z85>m/z57为56.9/11eV、m/z85>m/z43为58.1/11eV、裂解电压100V条件下,分别对杀草强残留量进行检测,其中液相色谱荧光检测器法杀草强的检出限为0.01mg/kg;液相色谱串联质谱法杀草强的检出限为0.005mg/kg。该方法简便、快速,分析结果准确、可靠,两种仪器条件可灵活选择,适用于食品中杀草强残留量的快速检测。更多还原

【Abstract】 High-performance liquid chromatography with fluorescence detection (HPLC-FLD) and high performance liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry (HPLC-APCI-MS/MS) were used for analyzing amitorle residue in plant-derived foodstuffs.In HPLC-FLD analysis,the excitation wavelength and emission wavelength were set at 380 and 484 nm,respectively.HPLC-APCI-MS/MS analysis was carried out in the MRM mode under the conditions as follows:parent ion,m/z 85;quantitative daughter ion,m/z 43;qualitative daughter ion,m/z 57;collision energy,m/z 85 > m/z 57 to 56.9/11 eV,m/z 85 > m/z 43 to 58.1/11 eV;and cracking voltage,100 V.Limit of detection was 0.01 mg/kg for HPLCFLD method and 0.005 mg/kg for HPLC-APCI-MS/MS method.Both methods demonstrate the advantages of simplicity,rapidity,accuracy and reliability and are alternatives to each other for the determination the determination of amitorle residue in plant-derived foodstuffs.更多还原