【摘要】 研究一种能够同时测定水产品中21种磺胺类药物残留量的检测方法——超高效液相色谱质谱法。样品经乙酸乙酯提取,经固相萃取小柱净化,氮气吹干后,用1mL流动相定容残余物。采用ACQUITYTM BEH C18色谱柱,用甲醇和5mmol/L乙酸铵溶液(含体积分数0.1%甲酸)作流动相,采用质谱技术分析。结果表明,检出限为0.01～0.20μg/kg,定量限为0.03～0.67μg/kg,平均回收率为68%～82%,相对标准偏差为4.1%～11.8%。该方法适合水产品中多种磺胺类药物的检测。更多还原

【Abstract】 An ultra performance liquid chromatography-electrospray ionization tandem mass spectrometric (UPLC-MS/MS) method has been developed for the simultaneous determination of 21 sulfonamide residues in aquatic products. Samples were extracted with ethyl acetate, cleaned up using an HLB SPE cartridge and blown to dryness under nitrogen, and the residues were then dissolved in 1 mL of mobile phase. The 21 analytes were separated on ACQUITYTM BEH C18 column with a mobile phase composed of methanol and 5 mmol/L aqueous ammonium acetate solution (containing 0.1% formic acid). The limits for qualitative detection and quantitative determination were 0.01 -0.20μg/kg and 0.03 -0.67 μg/kg, respectively. The average recoveries at three spike levels ranged 68%-82%, with RSDs ranging 4.1%-11.8%. All these figures of merit demonstrate a good suitability of this method for the simultaneous determination of sulfonamide residues in aquatic products.更多还原