【摘要】 为了增强纯生啤酒的泡沫性能,从酿酒酵母表达质粒YEplac181出发,将大麦脂转移蛋白1(LTP1)成熟肽的编码序列置于酿酒酵母ADH1启动子(alcohol dehydrogenase promoter)和CYC1终止子(cytochrome C terminator)的调控下,构建大麦脂转移蛋白1的酿酒酵母表达质粒YEp181-KAMLC。通过酿酒酵母α-信息素信号肽的引导分泌,酿酒酵母表达的成熟大麦LTP1被分泌到发酵液中。对发酵液的检测表明,在发酵132h后LTP1的产量可达到29.45mg/L。更多还原

【Abstract】 In order to enhance the foam stability of green draft beer,barley lipid transfer protein 1(LTP1) encoding DNA sequence from Hordeum vulgare was modified to construct yeast Saccharomyces cerevisiae expression plasmid YEp181-KAMLC of barley LPT1 using the plasmid YEplac181.The expression of barley LTP1 was regulated by alcohol dehydrogenase promoter(ADH1 promoter) and terminator cytochrome C terminator(CYC1) of Saccharomyces cerevisiae,and the matured form of barley LTP1 was secreted under the lead of α-mating factor 1 signal peptide.Fermentation for 132 h at 30 ℃ gave a yield of barley LTP1 as high as 29.45 mg/L.更多还原