【摘要】 以碱性蛋白酶做催化剂水解玉米蛋白粉,水解产物精制后,采用SephedaxG-15和G-10葡聚糖凝胶层析法及高效液相层析法进行分离纯化。以α-生育酚为阳性对照,研究水解产物及其分离组分对DPPH自由基的清除活性和对亚油酸自氧化的抑制作用,同时测定它们的还原力。采用邻苯三酚自氧化法研究它们对超氧阴离子自由基的清除作用。结果表明,抗氧化活性最高的组分是经SephedaxG-10层析法分离得到的组分Ⅱ。组分Ⅱ的分子量分布范围在400～700D之间,富含谷氨酸、丙氨酸、亮氨酸、组氨酸、脯氨酸和苯丙氨酸。更多还原

【Abstract】 Corn gluten meal was hydrolyzed by proteolysis with alkaline protease and the refined hydrolysate was then separated and purified by Sephadex G-15 and G-10 column chromatographic and preparative HPLC methods.With α-tocopherol as a positive control,the hydrolysate and its fractions were evaluated for antioxidant activity by DPPH radical scavenging assay,linoleic acid auto-oxidation inhibition assay,ferric reducing/antioxidant power(FRAP) assay and superoxide anion scavenging assay based on pyrogallol auto-oxidation method.Results revealed that fraction II eluted from Sephadex G-10 column exhibited the highest antioxidant activity,a relative molecular mass distributed in range of 400 to 700 D detected by MS and an amino acid composition mainly consisting of glutamic acid,alanine,leucine,histidine,proline and phenylalanine.更多还原