【摘要】 分析比较不同提取方法对天麻药材中活性成分提取的影响。以天麻生药材为分析对象,比较微波辅助提取、超声波提取、冷凝回流提取3种提取方法对天麻素提取率的影响。采用高效液相(HPLC)测定其提取的天麻素含量,条件为AngilentTc-C18(4.6mm×250mm,5μm)色谱柱,V(乙腈)∶V(0.05%磷酸)=15∶85为流动相,流速0.5mL/min,检测波长221nm,柱温25℃。天麻素在4.28～214μg范围内线性关系良好,平均回收率为100.02%(RSD=1.52%)。微波辅助提取法提取天麻素得率为1.613%,超声波法提取天麻素得率为1.215%,冷凝回流提取天麻素得率为0.703%。对于天麻生药材,不同提取方法对活性成分天麻素的提取影响较大,建议选用微波辅助提取法作为天麻素的提取方法。本实验所建立的HPLC方法具有良好的精密度、重现性,结果准确可靠,可作为天麻药材及天麻素类产品的质量控制方法。更多还原

【Abstract】 The effects of the gastrodine content of the active component in Gastrodia elata were compared and analyzed by different extracting methods.The raw Gastrodia elata was used and the concentration of gastrodine determined by HPLC after three extracting techniques.HPLC method was performed on as the mobile phase was a mixture of acetonitrile-0.05% phosphoric acid ( v∶ v = 15∶85) at 25℃,the chromatographic column was Agilent Tc-C18( 416mm × 250mm,5μm) ,the flow rate was 0.5mL/min,and the detection wavelength was 221nm.The calibration curve was linear( R2 = 0.9998) in the range of 4.28 ～ 214μg for gastrodin,the average recovery was 100.02% with RSD 1.52%.The ratios of gastrodine was 1.613% by the microwave extracting method,the ratios of gastrodine were 1.215%,0.703% respectively by ultrasonic extracting method and circumfluence extracting method extraction,which was higher than other extracting methods by raw material. Different methods have evident extracting effects on the gastrodine with raw material. According to active component most suitable microwave extracting method should be adopted.The HPLC method built by this study is stable,reliable,and which can be used as a quality control item for Gastrodia elata and the gastrodine products.更多还原