【摘要】 通过单因素实验选出屎肠球菌(Enterococcus faecium)BC-3产类细菌素发酵培养基最佳碳源为蔗糖,氮源为牛肉膏+蛋白胨+酵母膏,刺激因子为吐温80;采用Plackett-Burman设计法对选取的10个相关影响因素进行了评价,筛选出了3个主要影响因素分别为牛肉膏、蛋白胨、柠檬酸二铵,且3个因素在PB设计的水平范围内,对响应值的影响均为正效应。更多还原

【Abstract】 In the fermentation of Enterococcus faecium BC-3 produced bacteriocin-like substance experiment, single factor design was used to determine that sucrose was the best carbon source,beef extract,peptone and yeast extract were suitable nitrogen sources,and stimulating factor was tween 80.Subsequently,the methodology of Plackett-Burman design was undertaken to evaluate the influence of the ten related factors.Three statistically significant parameters including beef extract,peptone and dibasic ammonium citrate were selected,and all of them influenced bacteriocin-like substance production positively within designed level.更多还原