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用复性电泳技术研究金线鱼消化道蛋白酶的分布

Activity distribution of digestive proteases from Nemipterus Virgatus by non-denatured electrophoresis

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【作者】 朱美娟陶红

【Author】 ZHU Mei-juan1,TAO Hong2(1.Guangdong Industry Technical College,Guangzhou 510300,China;2.College of Light Industry and Food Sciences,Guangzhou 510641,China)

【机构】 广东轻工职业技术学院食品系华南理工大学轻工与食品学院

【摘要】 采用复性电泳和活力检测方法对3组不同体重的金线鱼(Nemipterus virgatus)消化道蛋白酶的分布的研究表明:金线鱼各消化器官蛋白酶活性均随实验鱼体重增加而上升,但与体重增加不成比例。不同体重的金线鱼的消化道蛋白酶不仅存在活力差异,在种类和数量上也存在明显差别。3组鱼的消化道蛋白酶单位酶活力从高到低依次为幽门盲囊>胃>前肠>中肠>后肠>肝胰脏,但以总活力计算时,仍以胃部蛋白酶的总活力最大。

【Abstract】 The distribution and properties of three groups of Nemipterus virgatus were studied by non-denatured electrophoresis. The results showed that the activities of protease in all digestive organs went up with body weight increasing,but there was not ratio between the activity and body weight. Not only the activity but also the species and amounts were different with the changing of body weight. The protease activity was in descending order,pylorus caeca>stomach>foregut>midgut>hindgut>hepatopancreas. But maximal total protelytic activity was found in stomach.

  • 【文献出处】 食品工业科技 ,Science and Technology of Food Industry , 编辑部邮箱 ,2010年02期
  • 【分类号】TS254
  • 【被引频次】3
  • 【下载频次】97
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