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猪肉复合酶解及其热反应产物挥发性成分分析研究

Study on hydrolysis of pork enzymatic hydrolysis by complex proteases and analysis of the volatile components of Maillard reaction products

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【作者】 沈晗孙宝国廖永红肖阳

【Author】 SHEN Han,SUN Bao-guo,LIAO Yong-hong ,XIAO Yang(School of the Chemical and Environmental Engineering,Beijing Technology and Business University,Beijing 100048,China)

【机构】 北京工商大学化学与环境工程学院

【摘要】 比较14种蛋白酶酶解猪肉水解度大小与其热反应产物风味,筛选出水解度较大,风味较好的Trypsin、Papain、Neutrase(powder)、Novozymes Flavorase、Novozymes Protamex、Alcalase 2.4L、Protex6L酶进行L18(37)正交实验,得水解度最大的复合酶组合为A3B3C3D2E3F2G3,其最适酶解条件为加酶量250U/g猪肉、60℃、自然pH、2.5h,GC-MS分析酶解物热反应肉味香精挥发性成分的主要风味物质为醇类和醛类,其中苯甲醛与糠醛与肉味关系密切。

【Abstract】 By comparing the DH and taste of the 14 proteases hydrolyzates of pork protein,Trypsin ,papain,neutral protease,Novozyme flavourzyme,Novozyme protamex,alcalase 2.4L and protex6L were selected to be tested in orthogonal experiment. The best combination of the biggest DH was A3B3C3D2E3F2G3,its optimum condition was protease quantity 250U/g pork,60℃,natural pH,2.5h. Maillard reaction products were analyzed by GC-MS,the flavor substances which mainly were alcohol,aldehydes and so on,furfural and benzaldehyde had a close relationship with the porcine flavor.

【关键词】 蛋白酶酶解水解度热反应GC-MS
【Key words】 proteaseenzymatic hydrolysisDHMaillard reactionGC-MS
【基金】 “十一五”国家科技支撑计划项目(2007BAK36B05)
  • 【文献出处】 食品工业科技 ,Science and Technology of Food Industry , 编辑部邮箱 ,2010年02期
  • 【分类号】TS264.3
  • 【被引频次】6
  • 【下载频次】467
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