【摘要】 AP-2β是转录因子AP-2家族中的重要一员.AP-2是一个与哺乳动物的发育、细胞生长、分化、凋亡和肿瘤发生都有密切联系的重要的转录因子家族.最近的研究表明,AP-2能通过与其他蛋白的相互作用来调控AP-2下游基因的转录活性.采用免疫共沉淀结合质谱鉴定的方法筛选与AP-2β相互作用的蛋白.结果筛选到Stomatin like protein2(SLP-2)蛋白可能与AP-2β相互作用.免疫共沉淀实验证实外源过表达和内源的SLP-2蛋白都能与AP-2β蛋白相互作用.而且,细胞免疫荧光实验发现外源表达的Myc-AP-2β蛋白与内源的SLP-2蛋白在MCF-7细胞的胞质中有共定位.此外,免疫印记实验结果表明过表达SLP-2能上调AP-2β的蛋白水平,这就表明SLP-2与AP-2β相互作用后,SLP-2可能使AP-2β蛋白更稳定.这些研究结果为进一步研究这两个基因的功能提供了新的切入点.更多还原

【Abstract】 AP-2β is an important member of transcription factor AP-2 family.AP-2 is a transcription factor implicated in mammalian development,cell proliferation,apoptosis and carcinogenesis.Recent studies have shown that interaction partners can modulate the transcriptional activity of AP-2 over the downstream targets.To identify potential AP-2 β interacting partners,we screened AP-2 β immunocomplexes by mass spectrometry and identified SLP-2 as a putative AP-2 β interacting protein.It is demonstrated that both exogenously expressed and endogenous SLP-2 interacted with AP-2 β in vivo by co-immunoprecipitation assays.In addition,indirect immunofluorescent analyses demonstrated that AP-2 β and SLP-2 co-localized in the cytoplasm of the MCF-7 cell lines.Furthermore,over-expression of SLP-2 enhanced the protein expression level of AP-2 β.Thus,through protein-protein interaction,SLP-2 can stabilize AP-2 β.These results provided a new clue for further research on the AP-2β and SLP-2 function.更多还原