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采用3,5-二硝基水杨酸比色法测定饲料总淀粉含量

Determination of the content of total starch in feed by colorimetric method with 3,5-dinitrosalicylic acid

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【作者】 马晶晶王际英张利民王世信黄炳山孙永智

【Author】 Ma Jingjing,Wang Jiying,Zhang Limin,et al.

【机构】 山东省海洋水产研究所

【摘要】 采用3,5-二硝基水杨酸(DNS)比色法测定饲料样品中总淀粉含量。结果表明,该方法标准曲线在0.2~1.2mg范围内,线性关系良好,回归方程Y=0.7442X-0.0465,相关系数r=0.9998,平均加标回收率为96.8%~98.1%。实验表明,DNS比色法测定总淀粉含量简单易行,且重复性好,可作为饲料中总淀粉含量的常规测定方法。

【Abstract】 Colorimetric analysis of total starch content in the diet using 3,5-dinitrosalicylic acid(DNS)at the wavelength of 540 nm was clarified in the paper.Results showed that glucose within the levels of 1.2 mg was linearly correlated with absorbency values(Y=0.7442X-0.0465,r=0.9998),and the average recovery rate ranged from 96.8 % to 98.1 %.It was concluded that the above method could be conventionally used for total starch content determination in feeds.

【关键词】 饲料总淀粉3,5-二硝基水杨酸
【Key words】 feedtotal starch3,5-dinitrosalicylic acid
  • 【分类号】S816.17
  • 【被引频次】20
  • 【下载频次】736
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