【摘要】 目的以细胞因子(肿瘤坏死因子α和白细胞介素1β)为研究对象,对国产无机三氧化物聚合物(MTA)介导的炎症反应进行相关的免疫学评价。方法运用双抗夹心ELISA检测新材料国产MTA引起体外小鼠的单核巨噬细胞系RAW264.7细胞TNF-α和IL-1β的变化。结果未经脂多糖诱导的RAW264.7细胞与阳性材料接触后,TNF-α和IL-1β的表达均高于阴性材料,差异均有显著性意义(P<0.05);国产MTA组和进口MTA组TNF-α和IL-1β的表达与阴性材料比较差异无显著性意义(P>0.05);经脂多糖诱导的RAW264.7细胞与阳性材料、国产MTA、进口MTA接触后,TNF-α和IL-1β的表达均高于阴性材料,差异均有显著性差异(P<0.01)。结论国产MTA具有良好的生物相容性。更多还原

【Abstract】 Objective To conduct an immunological evaluation on the inflammatory reaction mediated by the domestic mineral trioxide aggregate(MTA),with tumor necrosis factor α(TNF-α)and interleukin 1β(IL-1β)as indices.Methods The sandwich ELISA is used to determinate the TNF-α and IL-1β in the macrophages reacted with the domestic MTA.Results Macrophages stimulated with positive materials produce extremely significant more TNF-α and IL-1β compared to negative materials(P<0.05).There are no significant differences in TNF-α and IL-1β between the domestic MTA,imported MTA and negative materials(P>0.05);Stimulated with positive materials,the domestic and imported MTA the lipopolysaccharide-induced macrophages produce more TNF-α and IL-1β compared to negative materials,with extremely significant differences(P<0.01).Conclusion Domestic MTA shows good biocompatibility.更多还原