【摘要】 目的研究表皮生长因子受体(epidermal growth factor receptor,EGFR)在不同转移潜能乳腺癌细胞中的表达,并探讨其在乳腺癌侵袭转移过程中的作用。方法利用人工基质膜侵袭实验获得高、低转移潜能乳腺癌细胞亚系,四甲基偶氮唑盐(MTT)法检测两系细胞生长曲线和倍增时间,流式细胞仪检测两系细胞周期,transwell侵袭小室模型比较两系的迁移能力。应用逆转录聚合酶链反应(RT-PCR)和免疫印迹(Western Blot)检测EGFR在两系细胞中的表达。结果利用transwell小室成功筛选出高、低转移潜能乳腺癌细胞亚系;它们的体外生长速度、倍增时间、细胞周期和侵袭力具有明显差异;RT-PCR和Western Blot均显示在高转移潜能乳腺癌细胞中EGFR在基因和蛋白水平的表达均显著高于低转移乳腺癌细胞。结论 EGFR的过表达与乳腺癌细胞侵袭能力显著性相关,EGFR在乳腺癌侵袭过程中发挥了重要的作用。更多还原

【Abstract】 Objectives To detect the expression of epidermal growth factor receptor( EGFR) in human breast cancer cell lines with different metastatic potentiality,and to investigate the effect of EGFR on metastasis of breast cancer in human.Methods Vitro invasion assays in matrigel was applied to screen two different metastatic potentiality sub-clones of human breast cancer cell lines.MTT assay was applied to detect cell growth curve and doubling time;Flow cytometry was used to test cell cycle;Transwell assay was applied to observe the cell motility and invasiveness;RT-PCR and Western blot were used to detect the gene and protein expressions of EGFR in human breast cancer cell lines with different metastatic potentiality.Results Two sub-clones of cells with high-metastatic and low-metastatic potentiality were obtained through continuous in vitro invasion assay.There were significant differences between high-metastatic potentiality cells and low-metastatic potentiality cells in cell proliferation,doubling time,cell cycle and invasion ability.The expression level of EGFR in mRNA and protein was significantly higher in cells with high-metastatic potentiality than in those with low-metastatic potentiality.Conclusions The over-expression of EGFR is highly related with metastatic potentiality of human breast cancer cells.EGFR may play an important role in malignant progression of human breast cancer.更多还原