【摘要】 目的对酶联免疫吸附试验(ELISA)、时间分辨免疫荧光法(TRFIA)、化学发光法(CLIA)、电化学发光法(ECLIA)检测乙型肝炎血清标志物[乙型肝炎表面抗原(HBsAg)、乙型肝炎表面抗体(抗HBs)、乙型肝炎e抗原(HBeAg)、乙型肝炎e抗体(抗HBe)、乙型肝炎核心抗体(抗HBc)]的结果进行分析,评价不同方法学检测结果之间是否具有一致性。方法用ELISA、TRFIA、CLIA、ECLIA分别检测100例乙型肝炎病毒(HBV)感染者和50名正常体检者的乙型肝炎血清标志物。以ECLIA作为参考方法,通过Kappa检验评价不同方法学检测结果之间的一致程度。结果 4种方法检测乙型肝炎血清标志物的结果具有高度以上一致性,CLIA和ECLIA在检测敏感性上更有优势。结论目前广泛使用的检测乙型肝炎血清标志物的方法具有较好的一致性,为实验室结果互认提供了依据。其中ELISA适用于人群初筛,CLIA和ECLIA对于肝炎患者疗效判断更有价值。更多还原

【Abstract】 Objective To evaluate the method concordance of enzyme-linked immunosorbent assay(ELISA),time-resolved fluorescence immunoassay(TRFIA),chemiluminescence immunoassay(CLIA) and electrochemiluminescence immunoassay(ECLIA) for detecting Hepatitis B vivus(HBV) markers [hepatitis B surface antigen(HBsAg),antibody to HBsAg(anti-HBs),hepatitis B e antigen(HBeAg),antibody to HBeAg(anti-HBe) and antibody to hepatitis B core antigen(anti-HBc)] in serum.Methods HBV markers in sera of 100 patients infected with HBV and 50 healthy subjects were detected by ELISA,TRFIA,CLIA and ECLIA.ECLIA was performed as reference method,and Kappa test was used for determining the concordance between these different methods.Results It showed high concordance between the 4 methods.CLIA and ECLIA had better sensitivity than the others.Conclusions ELISA is a routine method with good concordance for screening HBV markers.It provides the reference for laboratory mutual recognition,and it is suitable for primary screening.CLIA and ECLIA methods have much value in evaluating therapeutic effect of hepatitis patients.更多还原