【摘要】 以罗汉果子叶为受体,通过农杆菌介导法,将从拟南芥中克隆到的NPR1基因导入罗汉果。经抗生素筛选、PCR检测和Southern杂交分析,结果表明:NPR1基因已经整合到罗汉果基因组。通过外植体不同分化阶段的抗性筛选,获得转基因植株。在烟草花叶病毒接种6周后,转基因植株的发病程度明显低于非转基因植株。更多还原

【Abstract】 The cotyledon of Siraitia grosvenorii was used as a receptor,and the NPR1 gene cloned from Arabidopsis thaliana was transferred to S.grosvenorii by Agrobacterium-mediated transduction. The antibiotics screening,PCR testing and Southern blot hybridization showed that the NPR1 gene was integrated into S.grosvenorii genome.The transgenic plant was obtained by resistant screening at different differentiation phases of the explants.The test indicated that the transgenic plant inoculated with tobacco mosaic virus(TMV) was significantly lower in disease severity after six weeks than the non-transgenic plant.更多还原