【摘要】 目的:构建人体内牙菌斑生物膜模型,观察不同时间自然形成菌斑生物膜的特点,体外观察精油成分漱口液对生物膜的渗透及杀菌效果。方法:按纳入标准选取6名志愿者,将刻有凹槽的羟基磷灰石圆片固定于活动夹板,配戴夹板,分别于6、24、48h取出圆片,于激光共聚焦显微镜下观察生物膜结构,检测总生物膜厚度,以及其中活菌厚度(μm)和荧光强度百分比(%)。观察各个时期形成的生物膜经漱口液作用1、5、15和30min后生物膜中活菌厚度及荧光强度的变化。应用SPSS13.0软件包进行随机区组单因素方差分析,SNK法进行均数两两比较。结果:6、24、48h形成的菌斑生物膜厚度分别为(11.92±4.63)μm、(18.63±4.66)μm和(27.55±6.35)μm,48h内菌斑生物膜逐渐增厚(P<0.05),尤以6h内增厚速度最快。不同时间形成的菌斑生物膜中,活菌厚度、荧光强度无显著差异(P>0.05)。6h形成的生物膜经漱口液作用,5min后活菌厚度和荧光强度即明显降低,15min后降至最低(P<0.01);24h和48h的生物膜则在漱口液作用15min后出现活菌厚度和荧光强度明显降低(P<0.05)。结论:本实验建立了体内收集自然形成完整的菌斑生物膜模型,观察到菌斑生物膜在48h内逐渐增厚、成熟。精油成分漱口液对不同时间形成的菌斑生物膜具有良好的渗透和抑菌作用,提示该模型可用于自然形成完整牙菌斑生物膜的研究。更多还原

【Abstract】 PURPOSE:Human dental plaque is implicated in a number of oral diseases. Collection of its undisturbed intact structure facilitates observing the formation and the effect of treatment. This study established a model in vivo to collect intact natural dental plaque biofilm,and observed the features and evaluated the immediate penetration and bactericidal effect of an essential oil (EO) mouthrinse on it at different periods. METHODS:Three 500μm wide grooves were cut into hydroxyapatite (HA) discs. The discs were worn by six volunteers for 6,24 and 48 hours,then broken into halves,one served as control,while the other received a one-minute extra-oral EO mouthrinse treatment. 5,15 and 30 minutes later,the plaque was visualized with a vitality staining technique to observe the sustained changes of biofilm structure in situ and the effect of EO on intact biofilm. The biofilm thickness and fluorescent density of vital and total bacteria were obtained through diagram analysis,assessing the percentage of thickness and fluorescent density of vital to total bacteria. All data analysis was performed using SPSS 13.0 software package. One-way ANOVA and Student-Neuman-Keuls tests were used. RESULTS:The thickness of 6h,24h and 48h biofilm was (11.92±4.63)μm,(18.63±4.66)μm,(27.55±6.35)μm,respectively,which increased significantly within 48 hours (P<0.05),especially those within 6 hours. The percentages of thickness and fluorescent density of vital to total bacteria at different periods showed no significant changes (P>0.05). For 6-hour samples,plaque vitality for thickness and fluorescent density decreased significantly within 5 minutes after exposure to the EO (P<0.05). Meanwhile,for 24,48-hour samples,plaque vitality decreased significantly within 15 minutes (P <0.05). CONCLUSIONS:This study successfully establishes a model for collecting natural undisturbed plaque biofilm in situ,showing the changes constantly within 48 hours. EO mouthrinse has an immediate penetration and antimicrobial effect on the sustained dental plaque biofilm,especially 15 minutes later.更多还原