【摘要】 目的研究结缔组织生长因子(CTGF)反义寡核苷酸(ASODN)对人肾小管上皮细胞(HKC)转分化的影响。方法用巨噬细胞趋化因子-1(MCP-1)和马兜铃酸Ⅰ(AAⅠ)联合诱导HKC转分化模型。实验设空白对照组、模型对照组、PEI组、CTGFASODN10ng/mL组和CTGF ASODN100ng/mL组,分别采用WST-8法、流式细胞术、RT-PCR法、细胞免疫组织化学法检测不同浓度的CTGF ASODN对HKC转分化模型细胞增殖和细胞周期的影响,以及α-SMA mRNA表达和α-SMA、TGF-β1、Fibronectin(FN)蛋白表达的变化。结果 100ng/mL的CTGF ASODN对MCP-1和AAⅠ联合诱导HKC转分化细胞具有促进细胞增殖的作用,并可减少实验模型导致的细胞S期阻滞,降低α-SMA mRNA表达,减少α-SMA、TGF-β1和FN蛋白的表达。结论 CTGFASODN能够抑制MCP-1和AAⅠ导致的人HKC转分化。更多还原

【Abstract】 Objective To investigate the effects of antisense oligodeoxynucleotide(ASODN)targeting connective tissue growth factor(CTGF)on transition of human kidney epithelial cells(HKC).Methods Transition models of HKC were induced by monocyte chemotactic protein-1(MCP-1)and aristolochic acid Ⅰ(AAⅠ).HKC were randomly divided into blank control group,model control group,PEI group,CTGF ASODN 10 ng/mL group and CTGF ASODN 100 ng/mL group.The effects of CTGF ASODN on proliferation and cell cycle of transition models of HKC were evaluated by WST-8 method and flow cytometry,respectively.The expression of α-smooth muscle actin(α-SMA)mRNA and α-SMA,Fibronectin(FN)and TGF-β1 protein of transition models of HKC were detected by RT-PCR and immunohistochemistry,respectively.Results 100 ng/mL CTGF ASODN reversed the proliferation inhibition,reduced S phase arrest,decreased the expression of α-SMA mRNA and α-SMA,FN and TGF-β1 protein of HKC induced by MCP-1 and AAⅠ.ConclusionCTGF ASODN can inhibit the transition of HKC induced by MCP-1 and AAⅠ.更多还原