【摘要】 目的探讨测定人肝癌细胞BEL-7404内芥酸甲酯浓度的方法。方法采用薄层色谱—气相色谱法测定空白细胞裂解液、加药细胞裂解液、加药细胞培养液内芥酸钾酯浓度,并确定芥酸甲酯浓度和峰面积之间的关系。结果芥酸甲酯50~5 000μg/ml具有良好的峰面积—浓度线性关系(r=0.999 95);芥酸甲酯微乳液进入到人肝癌细胞BEL-7404内的量为24.9%。结论薄层色谱—气相色谱法能够准确测定药物进入细胞的浓度。其操作简单快速、干扰小。更多还原

【Abstract】 Objective To investigate the method for the determination of concentration of erucic acid methyl ester in human hepatocellular line BEL-7404.Methods The concentration of erucic acid methyl ester in the blank cell lysate,the medium and the lysate of cells that added erucic acid methyl ester were detected by thin layer chromatography-gas chromatography(TLC-GC),and the relationship between the concentration of erucic acid methyl ester and the peak area.Results A good linear correlation between peak area and concentration were obtained within a concentration range of 50~5 000 μg/ml(r=0.999 95).The amount of erucic acid methyl ester microemulsion got into human hepatocellular line BEL-7404 were 24.9%.Conclusions TLC-GC can determine the concentration of the drug in the cell accurately;this method is simple,rapid and stability.更多还原