【摘要】 目的探讨Tadenan对早期糖尿病大鼠膀胱组织中神经生长因子(NGF)及P物质表达的影响。方法将36只雄性Wistar大鼠随机分为对照组(10只)、糖尿病组(10只)、治疗组(10只)、药物组(6只)。糖尿病组和治疗组大鼠先制作成糖尿病模型。饲养4周后,治疗组及药物组大鼠给予Tadenan 100 mg/(kg.d),溶于2 ml花生油中经胃管注入;对照组及糖尿病组大鼠给予同等剂量花生油赋形剂。以上动物共饲养8周。8周后处死,完整切取膀胱组织。采用RT-PCR及免疫组化法、ELISA法检测膀胱组织中NGF、P物质。结果糖尿病组NGF、P物质明显降低(P均<0.05);与糖尿病组相比,治疗组NGF及P物质显著升高(P<0.05)。结论 Tadenan可增加膀胱组织中NGF及P物质表达,推测这是Tadenan对糖尿病膀胱具有保护作用的机制。更多还原

【Abstract】 Objective To investigate the effects of Tadenan on nerve growth factor(NGF) and substance P in the bladder 4 weeks after diabetes induction.Methods 36 Wistar rats were divided into four groups: control group(n=10),diabetic group(n=10),treated group(n=10,diabetic rats were fed with Tadenan),drug group(n=6,normal rats were fed with Tadenan).Animals in diabetic group and treated group were successfully induced into diabetic model.4 weeks later,treated group and drug group were given Tadenan 100 mg/(kg·d),control group and diabetic group were given the same dose of oil for control.All the rats were fed 8 weeks.8 weeks later,the bladders were dissected.Its were used by RT-PCR and ELISA to evaluate the expression of NGF,and used immunohistochemistry and ELISA to detect the expression of substance P.Results Compared with the diabetic group,the expression of NGF and substance P were greatly increased in the treated group(P<0.05).Conclusions Tadenan increase the express of NGF and substance P,that is suspected is mechanism of Tadenan protecting.更多还原